Canadian Patents Database / Patent 2016948 Summary

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(12) Patent Application: (11) CA 2016948
(54) English Title: POLYSACCHARIDE MIXTURE WITH IMMUNE STIMULATING AND ANTI-PROLIFERATING EFFECT, METHOD OF PRODUCTION AND MEDICINES CONTAINING THE SUBSTANCES
(54) French Title: MELANGE DE POLYSACCHARIDES AVANT UN EFFET IMMUNO-STIMULANT ET ANTI-PROLIFERATION, METHODE DE PRODUCTION ET MEDICAMENTS LE CONTENANT
(52) Canadian Patent Classification (CPC):
  • 167/199
  • 260/225
(51) International Patent Classification (IPC):
  • C08B 37/00 (2006.01)
  • A61K 31/715 (2006.01)
(72) Inventors (Country):
  • OZEL, HUSEYIN Z. (Turkey)
  • BASER, KEMAL H. C. (Turkey)
  • CARBIK, ISMAIL (Germany)
  • WAGNER, HILDEBERT (Germany)
  • OZEL, HUSEYIN Z. (Turkey)
  • BASER, KEMAL H. C. (Country Unknown)
  • CARBIK, ISMAIL (Germany)
  • WAGNER, HILDEBERT (Germany)
(73) Owners (Country):
  • OZEL, HUSEYIN Z. (Country Unknown)
  • BASER, KEMAL H. C. (Country Unknown)
  • CARBIK, ISMAIL (Country Unknown)
  • WAGNER, HILDEBERT (Country Unknown)
  • OZEL, HUSEYIN Z. (Turkey)
  • BASER, KEMAL H. C. (Not Available)
  • CARBIK, ISMAIL (Not Available)
  • WAGNER, HILDEBERT (Not Available)
(71) Applicants (Country):
(74) Agent: MALCOLM JOHNSTON & ASSOCIATES
(45) Issued:
(22) Filed Date: 1990-05-16
(41) Open to Public Inspection: 1990-11-16
(30) Availability of licence: N/A
(30) Language of filing: English

(30) Application Priority Data:
Application No. Country Date
P 39 15 928.0 Germany 1989-05-16
P 40 01 756.7 Germany 1990-01-22

English Abstract



ABSTRACT/SUMMARY

The invention relates to new polysaccharides with immune stimulating
and antiproliferating effect from plants of the family Nerium
oleander. One of the polysaccharides is made up of .alpha.(1?4) bonded
D-galacturonic acid units and shows a molecular weight of from 30,000
to 40,000 D. Additionally, a lower molecular polysaccharide mixture
of approximately 3000 to 12000 D, having three lesser fractions of
varying molecular weights and content, was isolated.


Note: Claims are shown in the official language in which they were submitted.


THE EMBODIMENTS OF THE INVENTION IN WHICH AN EXCLUSIVE PROPERTY OR PRIVILEGE
IS CLAIMED ARE DEFINED AS FOLLOWS:
1. Polysaccharide of the general formula:
Image
in which the residual R demonstrates an average methylization
degree of approximately 90% and otherwise indicates,
independently of each other, hydrogen, alkali or earth alkalation
and which has a molecular weight of 30,000 to 40,000 D,
demonstrated through HPLC-gelpermeation chromatography.

2. Polysaccharide having a molecular weight of approximately 10000
to 12000 D, determined by HP-GPC, containing the sugars rhamnose,
arabinose, galactose and glucose in a molar relationship of 1.1 :
4.4 : 3.2 : 1.0, where glucose = 1Ø

3. Polysaccharide having a molecular weight of approximately 5000 to
6000 D, determined by HP-GPC, containing the sugars rhamnose,
arabinose, galactose and glucose in a molar relationship of 0.4 :
3.9 : 2.0 : 1.0, where glucose = 1Ø

4. Polysaccharide having a molecular weight of approximately 2500 to
3500 D, determined by HP-GPC, containing the sugars rhamnose,
arabinose, xylose, galactose and glucose in a molar relationship
of 2.0 : 0.9 : 0.3 : 0.9 : 1.0, where glucose = 1Ø

23



5. Method for producing a polysaccharide of claims 1 to 3,
characterized in that
(a) parts of the Nerium oleander tree are cooked in water for 2
to 4, preferably 2.5 to 3, hours;
(b) the solid particles are filtered off, the extract is cooled
at room temperature and filtered, the filtrate is dialyzed
against water with a molecular weight limit of approximately
10000D;
(c) the dialysis product is lyophilized, the lyophilized
material is treated with alcohol and the undissolved parts
are separated off;
(d) polysaccharides having molecular weights of approximately
3500 to 12000 D are isolated from the liquid using gel
chromatography.

6. Method for isolating a polysaccharide according to claim 5, in
which for step (b) the polysaccharide is separated out of the
filtrate, the precipitate is filtered off and dissolved in a
dissolving liquid and, if necessary, lyophilized, and then high
and low molecular fractions are obtained through gel filtration
and an acetic polysaccharide is obtained from the high molecular
fraction by ion exchange chromatography and elution with a salt
gradient.

7. Method according to claim 5 or 6, in which the solid pieces are
cooked in approximately 5 times as much distilled water.

8. Method according to claim 6, in which the polysaccharide in step
(b) is precipitated through addition of ethanol (96%) in a 1:1
ratio.

9. Method according to claim 8, in which the ethanol precipitation
is repeated twice.

24


10. Method according to one of claims 6 to 9, in which the acetic
polysaccharide is isolated by elution with 0.1 molar NaCl
gradients.

11. Pharmaceutical product containing at least one polysaccharide
according to one of claims 1 to 4 or a polysaccharide obtained by
one of the methods of claims 5 to 10 in combination with
acceptable pharmaceutical carriers and/or auxiliary materials.



A single figure which represents the drawing illustrating the invention.

For a clearer understanding of the status of the application/patent presented on this page, the site Disclaimer , as well as the definitions for Patent , Administrative Status , Maintenance Fee  and Payment History  should be consulted.

Admin Status

Title Date
(22) Filed 1990-05-16
(41) Open to Public Inspection 1990-11-16
Dead Application 1992-11-17

Payment History

Fee Type Anniversary Year Due Date Amount Paid Paid Date
Filing $0.00 1990-05-16

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Document
Description
Date
(yyyy-mm-dd)
Number of pages Size of Image (KB)
Representative Drawing 1999-08-02 1 3
Cover Page 1997-10-02 1 80
Abstract 1997-10-02 1 74
Claims 1997-10-02 3 190
Drawings 1997-10-02 1 9
Description 1997-10-02 21 1,573