Language selection

Search

Patent 2291974 Summary

Third-party information liability

Some of the information on this Web page has been provided by external sources. The Government of Canada is not responsible for the accuracy, reliability or currency of the information supplied by external sources. Users wishing to rely upon this information should consult directly with the source of the information. Content provided by external sources is not subject to official languages, privacy and accessibility requirements.

Claims and Abstract availability

Any discrepancies in the text and image of the Claims and Abstract are due to differing posting times. Text of the Claims and Abstract are posted:

  • At the time the application is open to public inspection;
  • At the time of issue of the patent (grant).
(12) Patent: (11) CA 2291974
(54) English Title: COMPOSITION COMPRISING PANAX PSEUDO GINSENG AND EUCOMMIAE ULMOIDES
(54) French Title: COMPOSITION COMPRENANT PANAX PSEUDOGINSENG ET EUCOMMIAE ULMOIDES
Status: Expired
Bibliographic Data
(51) International Patent Classification (IPC):
  • A61K 36/484 (2006.01)
  • A23K 10/30 (2016.01)
  • A23K 20/00 (2016.01)
  • A23L 33/105 (2016.01)
  • A61K 36/00 (2006.01)
  • A61K 36/258 (2006.01)
  • A61K 36/46 (2006.01)
  • A61P 1/16 (2006.01)
(72) Inventors :
  • SHA, SHINHAN (Japan)
  • YANAIHARA, NOBORU (Japan)
(73) Owners :
  • SHA, SHINHAN (Not Available)
(71) Applicants :
  • SHA, SHINHAN (Japan)
(74) Agent: GOWLING WLG (CANADA) LLP
(74) Associate agent:
(45) Issued: 2003-09-30
(22) Filed Date: 1999-12-10
(41) Open to Public Inspection: 2001-02-23
Examination requested: 1999-12-10
Availability of licence: N/A
(25) Language of filing: English

Patent Cooperation Treaty (PCT): No

(30) Application Priority Data:
Application No. Country/Territory Date
99116511.9 European Patent Office (EPO) 1999-08-23

Abstracts

English Abstract

A composition comprising " Panax pseudoginseng " and "Eucommiae Ulmoides", a composition comprising "gallic acid-containing herbal extracts and "Licorice Root" together with " Panax pseudoginseng " and "Eucommiae Ulmoides", a drink or food comprising the above composition, and medicines, food supplements, healthcare foods, mixing feed and feed for improvement of nutritional state, hypertension, high blood sugar, immune system and liver disorders.


French Abstract

Une composition comprenant « Panax pseudoginseng » et « Eucommiae Ulmoides », une composition comprenant des « extraits de plantes contenant de l'acide gallique » et de la « racine de réglisse » ainsi que « Panax pseudoginseng » et « Eucommiae Ulmoides », une boisson ou un aliment comprenant la composition ci-dessus, et des médicaments, compléments alimentaires, des alicaments, des mélanges d'aliments et des aliments pour l'amélioration de l'état nutritionnel, l'hypertension, de l'hyperglycémie, des troubles du système immunitaire et hépatique.

Claims

Note: Claims are shown in the official language in which they were submitted.




Claims

1. A composition for oral consumption comprising Panax pseudoginseng and
Eucommiae Ulmoides.

2. The composition mentioned in Claim 1 further com-
prising gallic-acid-containing herbal extracts and
Licorice Root.

3. The composition mentioned in Claim 1 in the form of
extracts of Panax pseudoginseng and Eucommiae Ulmoides.

4. The composition mentioned in Claim 2 in the form of
extracts of Panax pseudoginseng, Eucommiae Ulmoides, gallic
acid-containing herbal extracts and Licorice Root.

5. A medicine comprising the composition as defined in
any of the Claims 1 to 4.

6. The medicine mentioned in Claim 5 comprising a com-
position of Claim 1 or Claim 3 for healthcare and improvement
of nutritional state.

7. A medicine comprising the composition mentioned in
Claim 2 or Claim 4 for improvement of liver disorder.

8. The medicine mentioned in Claim 7 wherein said
liver disorder is hepatitis.

9. The medicine mentioned in Claim 8 wherein said
hepatitis is selected from the group consisting of hepatitis
C, chronic hepatitis and hepatitis B.



-2-

10. A food supplement comprising the composition men-
tioned in any of the Claims 1 to 4.

11. The food supplement mentioned in Claim 10 compris-
ing 10-80 weight % of- Panax pseudoginseng and Eucommiae
Ulmoides together, 4-20 weight % of gallic acid-containing
herbal extracts and 4-15 weight % of "Licorice Root".

12. A healthcare food comprising 0.1-20 weight % of the
food supplement mentioned in Claim 2, Claim 4, Claim 10 or
Claim 11.

13. The healthcare food mentioned in Claim 12, in the
form of powder, powder granule, tablet, drink and emulsion.

14. A mixed feed comprising the composition mentioned
in any of the Claims 1 to 4.

15. A feed comprising the mixed feed mentioned in Claim
14.

16. A method for the production of a composition of
Claim 1, which comprises obtaining condensed extracts of
Panax pseudoginseng and Eucommiae Ulmoides by condensing
hot-water extracts of minced dry Panax pseudoginseng and
Eucommiae Ulmoides and if necessary their pulverised product
by heat drying optionally obtaining condensed gallic acid-
containing herbal extracts by condensing hot-water extracts
of minced dry gallic acid-containing herbal extracts and if
necessary their pulverised products by heat drying; and
mixing and then drying extracts or pulverised products of
Panax pseudoginseng and Eucommiae Ulmoides extracts or
pulverised products of gallic acid-containing herbal extracts
and extracts or pulverised products of Licorice Root.


39

17. The composition, medicine, food supplement, healthcare food, mixed feed or
feed
mentioned in any one of claims 1 - 15, further comprising Siberian Solomonseal
Rhizome.

18. A composition comprising from 10-70% weight % of a water extract of Panax
pseudoginseng and from 10-70% weight % of a water extract of Eucommia ulmoides
further
comprising a water extract of a gallic acid containing herbal extracts and a
water extract of
Licorice root.

19. A medicine comprising the composition of claim 18.

20. A food supplement comprising the composition of claim 18.

21. The food supplement of claim 20 comprising from 20-76 weight % of a
combination
of water extracts of Panax pseudoginseng and Eucommia ulmoides; and from 4-20
weight %
of water extracts of gallic acid containing herbs; and from 4-15 weight % of
water extracts of .
Licorice root.

22. A healthcare food comprising 0.1.about.20 weight % of the food supplement
of claim
20.

23. The healthcare food of claim 22 in the form of powder, powder granule,
tablet, drink
or emulsion.

24. A mixed feed comprising the composition of claim 18.

25. A feed comprising the mixed feed of claim 24.

26. A method for producing the composition of claim 18 comprising:
obtaining condensed extracts of Panax pseudoginseng by heat drying hot water
extracts of minced dry Panax pseudoginseng;


40

obtaining condensed extracts of Eucommia ulmoides by heat drying hot water
extracts
of minced dry Eucommia ulmoides;
obtaining condensed extracts of gallic acid-containing herbs by heat drying
hot water
extracts of minced dry gallic acid-containing herbs;
obtaining extracts of Licorice root;
mixing the condensed extracts of Panax pseudoginseng, Eucommia ulmoides,
gallic
acid-containing herbs and extracts of Licorice root; and
drying the mixture.

27. The method of claim 26 wherein the extracts of Panax pseudoginseng contain
pulverized Panax pseudoginseng.

28. The method of claim 26 wherein the extracts of Eucommia ulmoides contain
pulverized Eucommia ulmoides.

29. A composition comprising from 30-90 weight % of a water extract of Panax
pseudognseng and from 10-70 weight % of a water extract of Eucommia ulmoides.

30. A composition comprising from 10-60 weight % of a water extract of Panax
pseudoginseng and from 40-90 weight % of a water extract of Eucommia ulmoides.

31. The composition of claim 29 whereby the water extract of Eucommia ulmoides
is 40
weight %.

32. The composition of claim 29 whereby the water extract of Panax
pseudoginseng is 30
weight %.

33. The composition of claim 29 whereby the water extract of Panax
pseudoginseng is 30
weight % and the water extract of Eucommia ulmoides is 40 weight %.


41

34. The composition of claim 30 whereby the water extract of Panax
pseudoginseng is 30
weight %.

35. The composition of claim 30 whereby the water extract of Eucommia ulmoides
is 40
weight %.

36. A method for producing the composition of claim 29 or 30 comprising:
obtaining condensed extracts of Panax pseudoginseng by heat drying hot water
extracts of minced dry Panax pseudoginseng; and
obtaining condensed extracts of Eucommia ulmoides by heat drying hot water
extracts
of minced dry Eucommia ulmoides;
mixing the condensed extracts of Panax pseudoginseng and Eucommia ulmoides;
and
drying the mixture.

37. The method of claim 36 wherein the extracts of Panax pseudoginseng contain
pulverized Panax pseudoginseng.

38. The method of claim 36 wherein the extracts of Eucommia ulmoides contain
pulverized Eucommia ulmoides.

39. The method of claim 26 wherein the extracts of gallic acid-containing
herbs contain
pulverized gallic acid-containing herbs.

40. The method of claim 26 wherein the extracts of Licorice root contain
pulverized
Licorice root.

Description

Note: Descriptions are shown in the official language in which they were submitted.


CA 02291974 2002-11-22
! . ~ 1
- 1 -
BACKGROUND OF THE INVENTION
Field of the Invention
The present invention relates to composition
com~5rising "~ Panax pseudoginseng '" and "Eucommiae Ulmoides
S (Eucommia Bark)', and, if necessary, comprising gallic acid
containing herbal extracts and "Licorice Root" additionally and
provides the method for-its production.
The composition of the present invention can be
used effectively not only for human healthcare and
improvement of nutritional state and immune system, but
also for therapy or prophylaxis against hepatitis and
infectious diseases as conventional or alternative
medicine and food supplement.
In addition, the composition of the present
invention can be used as food supplement not only for
protection against hypertension and diabetes mellitus of
animals such as dog, cat, pig, cattle, horse and others,
but also for healthcare and improvement of nutritional
state=and immune system of these animals including.bird
and fish.
Description of the Related Art
Results of a nationwide survey indicate a
marked increase in the number of individuals, who
suffered with the heavy weight, hypertension and diabetes
r.

CA 02291974 1999-12-10
- 2 -
mellitus, because of their environmental conditions, lack
of exercise and diet with high energy. Accordingly,
ability for protection against hepatitis, infectious
disease and immune disease will be markedly lowered.
These observations can be seen in animal's
world. In fact, pet animals kept in a narrow area of the
living rooms tend to suffer from disorder of nutritional
state, hypertension, psychosomatic stress and heavy
weight body with fat mass.
On the other hand, increase of patients
suffering from HBV and HCV will be a serious problem to
be solued.
In general, interferons have been used for
therapy of patients suffering from HBV and HCV, although
interferons had been known to have strong unfavorable
side effects.
SUMMARY OF THE INVENTION
It is an object of this invention to provide
food supplements and alternative-medicines having an
effect for significant improvements in symptoms such as high
cholesterol,-hypertension, diabetes mellitus and some of
cancers (in order to keep healthy condition).
A further object is to provide alternative
medicines which afford significant improvements in
-symptoms with HBV and HCV, when compared with therapy
with interferons.
The first objects of this invention have been

CA 02291974 2002-11-22
- 3 -
attained by providing food supplements and the related
products and alternative medicines comprising "Panax
pseudoginseng " and "Eucommiae Ulmoides' , and if
required, comprising gallic acid-containing herbal extracts
and "Licorice Root"
Still another object of this invention is to
provide alternative medicine comprising ' Panax pseudoginseng ~~
"Eucommiae Ulmoides", and if required,
comprising gallic acid-containing herbal extracts and
"Licorice Root", which are effective for therapy in symptoms
with various liver disorders.
Also provided herein are compositions
comprising '. Panax pseudoginseng " , "Eucommiae Ulmoides" ,
gallic acid-containing herbal extracts and "Licorice Root", which are
useful as food supplement for healthcare, alternative
medicine for therapy of liver disorders, supplemental
products, products for drink, animal's foods and food
compounds for animal, bird and fish.
The present invention provides a method for
production of the above-mentioned composition which
comprises obtaining condensed extracts of fanax pseudoginseng
and "Eucommiae Ulmoides" by condensing hot=water
extracts of minced dry "1 Panax pseudoginseng '" ~d
"Eucommiae Ulmoides", and if necessary, their pulverized ._
product by heat dry; obtaining condensed extracts of
w-gallic acid-containing herbal extracts by condensing hot-water
extracts of minced dry gallic acid-contain;ng herbal extracts and if
necessary their pulverised products by heat dry; and mixing and then

CA 02291974 2002-11-22
~ ~ !
- 4 -
drying extracts or pulverized products of " Panax pseudoginseng~"
and "Eucommiae Ulmoides", extracts or pulverized
products of gallic acid-containing herbal extracts and
extracts or pulverised products of "Licorice Root".
Alternative medicines, food supplements,
products for drinking and healthcare food for human and
animals comprising the above compositions are effective
for lowering blood, sugar and cholesterol and for
enhancing immune system.
In addition, the composition of the present
invention shows effect for lowering the elevated GOT and
GTP levels. Especially, oral administration of composions
eontai.ning gallic acid-containing herbal extracts resulted
in the reduction of elevated a-Fetoprotein and albumin in
human, suggesting that compositions of the present
invention may be effective for prevention or reduction of
the symptoms with high blood cholesterol, hypertension
and diabetes mellitus, and also "useful for prevention
against chroizic hepatitis. Although gallic acid-containing
herbal extracts themselves have been known to be difficult to
absorb into human or animal's body, compositions of this
invention comprising gallic acid-containing herbal extracts
can be readily absorbed into human and animal bodies.
25~-DESCRIPTION OF THE PREFERRED EMBODIMENTS
Panax pseudoginseng in thi$ invention consists
of the root of Panax pseudoginseng Wall . This Chinese

CA 02291974 2002-11-22
- 5 -
herb has been known to improve lipid metabolic failure,
and to control hypertension and pain relief.
Eucommia bark generally consists of dried bark
of Eucommiae ulmoides. This Chinese herb has been known
to reduce hypertension and high blood lipid level.
The Eucommiae Ulmoides in this invention,
however, not only refers to "Eucommia bark" but also
includes leaves and fruits of Eucommiae Ulmoides.
"Denhichi-Tochu-Sei" is a novel composition of
Chinese herbs comprising Panax pseudoginseng and
Eucommia bark, which was invented by the present
inventors, "Denhichi-Tochu-Sei" is produced by extracting
the root of Panax pseudoginseng and the Eucommiae
Ulmoides with hot water. The composition "Denhichi-
Tochu-Sei" can contain honey aid panax ginseng as
additives.
The word "Denhichi-Tochu-Sei" of the present
invention indicates the composition comprising dried
extracts of Chinese herbs, such as Panax pseudoginseng
and Eucommia mainly.
In "Donhichi-Tochusei" of the present invention
the compositions of Chinese herbs are Panax pseudoginseng
(Denhichi) 10-90 weight %, Eucommiae Ulmoides
(Tochu) 10-90 weight %,
Panax ginseng, 0-20 weight %, preferably 5-20
"weight %, honey 0-30 weight %. A preferable composition
for Denhichi-Tochu-Sei of the present invention is 30
weight % of Panax pseudoginseng , 40 weight % of

CA 02291974 2002-11-22
- 6 -
Eucomniae Ulmoides, 20 weight % of Panax ginseng and 10
weight .% of honey, or 30 weight % of Panax pseudoginseng ,
40 weight % of Eucommiae Ulmoides, 10 weight % of Panax
ginseng, 10 weight %of honey and 10 weight % of others.
In this invention, although most preferable
ratio of ranax pseudoginseng and Eucomrniae Ulmoides in
the'composition is 3:4, even at the 1:0.1- 1:9 ratio,
biological effects such as lowering blood pressure and
blood cholesterol and enhancing immune condition are
still retained.
Gallic acid is 3,4,5-trihydroxybenzoic acid, which is
contained in "Galla halepensis", "Gallae rhois", "Gallae
chinensis" and the like as Chinese medicines. The gallic
acid-containing herbal extracts include these Chinese
medicines.
Licorice root was obtained from the roots or
kernels of Glycyrrhiza glahra Linn.
Glycyrricine, which is a main component of
Licorice root, has -been known to improve several symptoms
with liver injuries and to elevate the serum transaminase
by the double blind examination.
- Farther, glycyrricine has been showed to
protect CC1,-induced liver injuries (Y. Ishii, Japan J.
Pahrmaco, 120, 71, 1971).
The composition (Denhichi-Tochu-Sei) and the
w composition containing Denhichi-Tochu-Sei, extracts of
Gallae rhois and Licorice root in the present invention,
dissolve in water and are readily absorbed into the living

CA 02291974 2002-11-22
7
body, and were found to afford the effects continuously.
Especially, granules or tablets obtained by
drying. under heating the composition comprising Gallae
rhois, Licorice root, "Denhichi-Tochu-Sei" and an
emulsifier show not only the effects of Gallae
rhois and Licorice root, but also the synergistic effect of
"Denhichi-Tochu-Sei't, ,improving the blood cholesterol levels,
blood sugar and liver functions.
The composition of this invention can further contain
"Siberian Solomonseal Rhizome".
Next, methods for'preparation of the
composition of this invention are described.
First, dried Eucommia (leaves, fruit, bark )
and dried Panax pseudoginseng were minced, and the
minced mixture was~extracted with hot water at 80~ -120
for 0.5-2 hr (extraction step). Then, through filtration
and concentration steps, the extracts thus obtained was
mixed with Panax Ginseng and honey (mixing step).
Further, by processing under hot wind, powdered Denhichi-
Tochu-Sei' are obtained.
In the same manner gallic acid-containing.herbal
extracts were obtained by extracting a.gallic acid-containing
Chinese herbe with hot water the minced dry Schisandrae, and
then concentrating the solution. The powder of gallic acid-
containing herbal extracts is obtained by heat drying the
extracts.

CA 02291974 2002-11-22
~ , a
-
Emulsifier was added to the above mixture to
prepare tablets under heat drying.
Gallic acid-containing herbal extracts and lycorice extract
can be contained with the above mentioned "Denhichi-Tochusei".
Addition of emulsifier to the mixture gave
tablets. By processing the composition as mentioned
above, powder, tablet, liquid and food supplements are
prepared, Honey, vegetable oil, xylitol or the like can
be used as emulsifier. The weight % of=the emulsifier is
preferably 0-20%.
The mixing ratio of "Denhichi-Tochusei", gallic acid-
containing herbal extracts and Licorice root is in a range of
10-80'%, 4-20 % and 4-15 % (weight %), respectively. In the
case of gallic acid-containing herbal extracts, a preferable
mixing range is 6-12 weight %. In case of Licorice, a
preferable mixing range is 6-11 weight % or 0 %, depending on
conditions of individual patients.
Especially compositions obtained by mixing
powdered Panax pseudaginseng ~ 60 weight %, "Denhichi-
Tochu-Sei" 20 weight %, gallic acid~containing herbal extracts 8
weight %, Licorice 8 weight % and emulsion 4 weight % are
favourable for production of various kinds of food supplements.
Compositions of the present invention can be
processed as powder, granules, tablets and dry syrup
suitable for alternative medicine, food supplements and'

CA 02291974 2002-11-22
1
_ g _
the like. Content of composition of the present
invention is in the range of 0.1-0.5 g per tablet, or in
the range of 0.1-30 weight %, preferably 1-5 weight %
based on alternative medicine or food supplements.
In case of tablets obtained by heat drying the
mixture of "Denhichi powder", "Denhichi-Tochu-Sei"_
gallic acid-containing,herbal extracts and Licorice, the content of
composition of this invention should be 0.25 g/tablet or less.
The proper amount of the forernentioned
"Denhichi-Tochu-Sei" for ingestion per day is 0.1-2 g/kg,
preferably 0.1-0.25g/kg.
In the present invention, pet includes animal,
bind and fish (dog, cat, monkey, rabbit, chicken, duck,
gold fish) which can be raised by human being.
, In addition, pig, cattle and horse (includes
racing horse) are included for the target.
In addition, composition of this invention can
be added to cakes, candy, bread, wine, liquor, curry and
any of health-care food.
Detailed explanations to this invention with
examples will be made below. Rats (Wister, male) and
mice (male) were used in the experiments.
Example 1 Analysis of "Denhichi-Tochu-Sei" (granule)
~- Table 1 shows the analytical data of "Denhichi-
Tochu-Sei" ( granule ) , which consists of panax pseudoginseng

CA 02291974 2002-11-22
- 10 -
30 weight %, Eucommia Bark 40 weight %, ginseng
20 weight % and honey 10 weight %.
In the following Examples, the composition of
this Example 1 was used as "Denhichi-Tochu-Sei"
(granule).

CA 02291974 1999-12-10
- 11 -
Table 1 Analysis Table of "Denhichi-Tochu-Sei" (granule)
Protein content 8.28% (w/w)



Sugar content 25.30% (w/w)



Elementary analysis value C . 40.95%


H . 6.68%


N 0.29%



Free amino acid: mg/lOOg granule Asp: 2.18


Ser: 2.74


Gly: 0.34


Ala: 0.28


Gln: 6.41


Ile: 0.65


Lys: 1.79


Table 2 shows the trace element contents of the
above-mentioned "Denhichi-Tochu-Sei" (granule) determined
by atomic absorption spectroscopy.

CA 02291974 1999-12-10
' - 12 -
Table 2 The results of trace element test of the above-
mentioned "Denhichi-Tochu-Sei" (granule)
Element a g/g Element a g/g


Na 25.0 Fe 7.63


51.0 Ca 304


Si 0.181 Water 8.88%


Sn <0.413 Ash content 0.42


Mg 526


Zn 4.39


Example 2 Effect of "Denhichi-Tochu-Sei" (granule) on
the blood serum cholesterol of rats with hyperlipemia
Rats (weight 180-200g) and mice (weight 18-20g)
were divided into four groups (10 rats and mice for each
group). The four groups were A) normal group, B) group
with hyperlipemia (prepared by high fat administration),
C) group with hyperlipemia (with high dosage of
"Denhichi-Tochu-Sei" (granule), and D) group with
hyperlipemia (with a low dosage of "Denhichi-Tochu-Sei"
(granule). In the C and D groups, Denhichi-Tochu-Sei and
high fat feed were administered in stomach through cannula
constantly for 30 days. One hour after the last
administration, blood sample was taken from rat
ophthalmic vein, from which serum was separated. The
serum was examined for the levels of cholesterol,
triglyceride, lipoprotein of low molecular weight, LDL

CA 02291974 1999-12-10
- 13 -
and lipoprotein of high molecular weight using Beckman's
serum biochemical analyzer CXS. The results are shown in
Table 3.
Table 3 clearly shows that addition of
Denhichi-Tochu-Sei (granule) to the high fat feed
prevents the appearance of hyperlipemia. Namely, blood
serum cholesterol and lipoprotein of lower molecular
weight were reduced and the slight elevation of
lipoprotein with higher molecular weight and reduction of
triglycerid were observed in group C and group D. In
fact, statistical and significant differences were
obtained between the values obtained with normal group
(A) or group (B) given only high fat feed and the values
obtained with groups given high dosage of "Denhichi-
Tochu-Sei" (referring to Table 3).

CA 02291974 1999-12-10
- 14 -
~


.
~ O~ N r-1


O O ~1 e-1 d' M N
+~ 3 ..,
ro


H ~ r-IO O O O


~ ~ \ -f-1 -f-1 -H ~-I
f.~ U
.~


O .~ N 01 C~ op
O lT
~-i


Cl~ W O In O 00 O~
-I r1


-i ..~ E . '
O a~


o~
a 3 E v o ~ o 0
3


* .O


fh N * *


er O W -i ~ O


O ~ O


~ O O


r-I O O



d1 r~O~ C~ 01 ri


~i - O O~ 1n a0 er


~


H O N ~-~1 N



*


.C
d' OD


r-I r1 ~' N


~ .~ 3


--o


r1 c~ n7


~ ~


o N


a o
_


E


a o ~ ~ ov o



G (-1 * * * U


~i O ~p r-1 CO M


r1 O r1 r1 O
tr
b


~ O O O O
O '''~
''~


G


E p



t~ ~ O O M ~ ~ *
~ ~


m E


a 3 E ~-~ 0 0 0
3


o b - ~~


o O * in * o~
s"'r


f-~O N r-I
O '


~
U r-I c .-~
~


N ~ O ~I N 'f'~


O \ ~ N


O O '~ rir1 er r1 00


O O ~n O~


O .~ E ~n O


V ~ ~ ~ O


E


..
U


x


m ~ \


o m


ro L1 v N r1


_
E



i


a~ ro


E


E


O 4-1 O o O O
~


2 O ro ~ .-i


O C ,C C


a~ a~ Cn a~
3


,~ ,~ r1 4-irI ,~ 4~
~ O ~
rl


_ r-i .~i ~ .ri o d cn ~r1 o .rl N
+~ r1 +~ ~
,C .-I


o cn ro tn ro .~ r~+~ cr .c +~
ro cn


r1 4-I 4-1 U ~ U W + ~ U U
N + U i


fly ro L~ i3~ CWrI'~'O ~L ~ TI ro
+~ O


O E O ,C O .~ ro .~N O .~ ro
C b 2i .~ b .O
~


O H O C~ O ~ u~ U +.~O tr~ N O .a-~
O U O G O U


i-1 O i~ ~ri H ~ri O O DC i-I O O 5C
U O O N r1 O
O


C7 Z C9 .~ C9 ,C b E O C9 ~d
~ 4-i 4.~ D ~ O
4-t E



CA 02291974 1999-12-10
- 15 -
Example 3 Effect of "Denhichi-Tochu-Sei' (granule) on
mice with deteriorated immune system.
Mice were divided into three groups, i.e., a
normal group for comparison, a group with deteriorating
immune system and a group with deteriorated immune
system given "Denhichi-Tochu-Sei" (granule).
A comparison of the immune system of these three groups was
made. As can be seen in Table 4, the immune system of
individual mouse seems to improve after administrating
~~Denhichi-Tochu-Sei" (granule).

CA 02291974 1999-12-10
- 16 -
E


a~



a~



a~


o m a


C wi N O


O O O O. C


a



a~ a o



0 0 0


o 0


~n 0


_ m
~ :a


0



m
m


x +'
~a


~ N


O O



ri
3



o u~ p,



~


Z m


b


- o


_


U


~ + O


O N O .ri



~ Zs b



-r~



U


_ ~ ~


~


.O '''i U O


O


T3 E'' o


N I v



.


w S.I O


-
.C


7~ >~


D


~


N-_


O O ro


,~ w


U ~' .C O


b ~ ~i


E1


~ O
3


c~ ,



a 0 ~


1 i O S
-rr .r


m


_



E N ,-I ~


o fa b ~ -.-i


~ 2


.7
C


z N

CA 02291974 1999-12-10
' - 17 -
Example 4 Fatigue durability test
Mice (weight 18-20g) were divided into two
groups. One is a normal group, the other is a group
given "Denhichi-Tochu-Sei' (granule) throughout for 20
days. The results of fatigue durability test, which was
conducted one hour after the last administration, are
shown in Table~5. In the test, a mouse carried an iron
with a weight corresponding to 10% of its body weight,
attached to its tail and was made swim in a thermostatic
pool of 40 cm length x 28 cm width x 30 cm depth at a
water temperature of 20'x. The time of swimming (sec)
was measured. The results are shown in Table 5.
Table 5 Swimming time of weighed mice given
"Denhichi-Tochu-Sei' (granule)
Group Number Dosage Swimming time


of


animals


( g/kg ( sec )
)


Normal group 10 60.42 18.81


Group with administration


of "Denhichi-Tochu-Sei


(granule= - 10 2 100.7231.91


*P<0.01
Table 5 clearly shows that the swimming time of
the group with administration of "Denhichi-Tochu-Sei" granule
was significantly longer than the normal group. The

CA 02291974 1999-12-10
- 18 -
results suggest that "Denhichi-Tochu-Sei' (granule) may
contribute to the durability for fatigue.
Example 5 Effect for mice with high blood sugar
Mice with blood sugar level of above 180 mg/dl
was used in this test. The mice (weight 21-23 g)
were divided into three groups (a normal mice group, a
group of mice with high blood sugar level and a group of
mice with high blood sugar level and administration of
"Denhichi-Tochu-Sei"- (granule). They were
examined for their blood sugar levels. The results are
shown in Table 5. After the mice with high blood sugar
level received "Denhichi-Tochu-Sei' (granule), the high
blood sugar level of mice lowered to normal level. The
results show that "Denhichi-Tochu-Sei' (granule)
possesses the ability effectively to control the blood
sugar level of mice suffering from diabetes.

CA 02291974 1999-12-10
- 19 -



a~



w


m o
cn


ao N


o ~ v


a~ -- _ ~ ,


~ U 0 '"~ N N


f-I +~ r
-1


H
C~ i.~~ C ~ 'C
i.~


i ''i ~
~


t tl~ i-1 ~' O
n E


U ~ v


V b C ,~, O .Q
cd ~



E_ ~ .b



D


.
O


w



,.
4'a


m O ~ 3


~ ' a


Gy o ,,.,~ ~ ~ N


O


ZT +~ O Cv ~ tn


ri ~ ~ O b C1


H ~


' ~ ~ N ~r-i~ t~ ~-1 ~
O


( f e-
/~ ~'-I E '' 1
~


r-I .r.i. p~ ! . . r1
'~


2! G ,~ N o ov 3
p



m o b ~ a~


r 0
-i


p N a1 cd


O


E' T1 E


U


O U ~


U ~ b


.o ,x w


N O
O


C D ~


~ O


0 r-I v


,S: Or


O


O


.C ~ O O O
4-~


V E ~"~ N N N
O


W



W


W


O


\O r~ b b a1
r1


O O O rt!
O


m - f-I O O U~
V~


r-) ' ~ r1 r-1 O


,a G .a .O b


fd O ,c;


U ~ ,C rtf
U


C1 C71 O


~rl ~ri +
E-~


C~ ~ ,C


r-~ ri
~i


O ,C ,C O
O .~


N +.>> -.~ ~U


~ri ~rl N O
O Ti


3 ~ 3 ~ -l
,~



a rd w cr a s~ a
m


a ~ ~ ro a ~s ro
A


o N o m o rn


N O t-i H ~ O~
O 4.~


C.~ Z C~ C~ N ..
v~ O



CA 02291974 1999-12-10
' - 20 -
Example 6 Effect of "Denhichi-Tochu-Sei", "Gallae rhois"
and "Licorice Root" (mixed extracts) for the
blood cholesterol of rats suffering with hyperlipemia
Rats and mice were divided into following four
groups (10 rats and mice for each group). They were A)
normal group, B) a group suffering from hyperlipemia
(with dosage of high fat feed) , C) a group suffering from
hyperlipemia (with high dosage of "Denhichi-Tochu-Sei",
"Gallae rhois" and "Licorice Root" (hereinafter
also referred to as mixed extracts), D) a group suffering
from hyperlipemia (with a small amount of dosage of
"Denhichi-Tochu-Sei", "Gallae rhois" and "Licorice
Root" mixed extracts).
The composition of the mixed extracts in this
example and the following examples was "Denhichi-Tochu-
Sei" 20 weight %, "Gallae rhois" 8 weight o and
"Licorice Root" 8 weight %. The C and D groups were
given the mixed extracts and high fat feed in stomach
through cannula constantly for 30 days. One hour after
the last administration, a blood sample was taken from
rat ophthalmic vein, from which serum was separated. The
serum=Was examined for the levels of cholesterol,
triglyceride, lipoprotein of low molecular weight, LDL
and lipoprotein of high molecular weight using Beckmann's
serum biochemical analyzer CX5. The results are shown in
Table 7.
It can be known from the results of Table 7
that the hyperlipemia was prevented when "Denhichi-Tochu-

,, CA 02291974 1999-12-10
' - 21 -
Sei", "Gallae rhois" and "Licorice Root" mixed
extracts were added to high fat feed. Namely, the
reduction of blood serum cholesterol, the reduction of
lipoprotein with low molecular weight, the slight
elevation of lipoprotein with higher molecular weight and
reduction of triglycerid was observed in the group C) and
group D). Statistical and significant differences were
obtained between the measurement value of normal group A)
or group B) with only high fat feed and the values of
groups C) and D) with the mixed extracts.

CA 02291974 1999-12-10
_ 22 _
'b


G
Ti S-t * O * * O


O ~ ~ o,~od, O ...i w
M N


~ \ O O O (C
O


p~ ,~ ~ ~ ~ O w
r1
f1 U
,~


~


o ~ ~ r
3


~ , G
0


~n a o ~' o 0


*
O b N O


O~ Ov O


r-I O O ~ ~ O


O


~ ~ Ui


f3~ ' O '~ w ~, O O
.. Q1 E ,",~


_ r1 ~ ~ tn GO r7 'C7


S-1


r1 [-~ O N ~-1 N !O


O E


x


~, ~ ch *
H


a7 ~ '..~o c~ o


.-fi d g


.~ a \ ~ of ri fy
b


a o N _ ~i-I +I o
r


E o o ao 0


p .~ r, ~n ~ Q,


~


a o ~-' ~' ' ' ,C
'


o +~


~ ~ s~ .-~


m m ,c ~ o rr.,
c~ ~,,


i ,-~1+~ oW-i .-. . ' . . 'C1


H ~ U O O ~ o 0


~ -


E


a a o m
o O 3


~ r
E,


E o . . o


a ~ o o v


a~
+~ ~ * m


,V O ~ ~*,~~ o a, o
U


t~ a~ ~ ' M y .; o


.N ~ O ~ N V
~


r-I 'f'~O +'I Ga
~


O . O O r1 d' O ~ *
s


23 O E m . o~ r


w V ~ ,-a op


i


T
E



N r1


O O a7


Q ~



U
r


~ o


0 0 0 0



a z


~ ~
z


Ei


w .c w ~ w


O +.~ O~ O m it O m
b Zi


_ c~ ,-I +~,-I .N
N O


c~ m w .C .~ U ,C N U
~ ~ O


w c~u~ c~ o~ or a~ ~da w 3 0~ ~d
w a~ ~d
w


E ~ ~ .~ c0 ~ ~ c~ f-t~ ~ O c~ f~
~ b .~ O N


o N o o+~ m v~a~o+~+~.c +~o.N+~ ~ u~ ~
~n x
x


N o ~ r~ .~ o ~ o ~ x s~ o ~ x
~ m .~ ~
cd c~


c9 2 a~ ~ 3 b ~ c9 + 'd a~c~ + 'v m
w 3 E 3 E
w w



CA 02291974 1999-12-10
' - 23 -
Example 7 Effect for hepatic diseases of "Denhichi-
Tochu-Sei', ~'Gallae rhois" and "Licorice Root"
mixed extracts
Table 8 shows the effect of "Denhichi-Tochu-
Sei", "Gallae rhois" and "Licorice Root" mixed
extracts for rats with hepatic disease (n=8) induced by
D-glucosamine ('800 mg/kg).
Table 8 Effect of "Denhichi-Tochu-Sei", "Gallae
rhois" and "Licorice Root" mixed extracts
(2000mg/kg) for rats with hepatic disease (n=8)
induced by D-glucosamine (800mg/kg)
Group with a dosage of
Normal group mixed extracts
GPT( L~~~"S. D) 1012'x'220 7301-17
GOT(11$~S. D) 1696'x'130 1252-35
Example 8 Effect of "Denhichi-Tochu-Sei", "Gallae
rhois" and "Licorice Root" mixed extracts for lipid
peroxidation: induced by carbon tetrachloride, lipid-
binding for 1'CC1,, CO production, NADPH oxidation and
oxygen consumption
Table 9 shows the effect on-lipid peroxidation
induced by carbon tetrachloride, lipid-binding for 1'CC1,,
CO production, NADPH oxidation and oxygen consuption.

CA 02291974 1999-12-10
' - 24 -



0


C
O a O

O O


O


U



.C v - .~


b ~ G
U "' a
E N
p U apt a b
b
z x
U
, p 0


~ x
b b o ~
o ,~ b . o
~, ' ~ o a"'
;i a b o
b b
a ~ a
~ b


m
o


O N p~~--I
b ~-plV O
U z
o ~ a ~ o c c o
a
w o b
~"'


'
O p U p
O m d
cd ~ O C .~.' ~
~ b
b , O
O O
a



o H 'F",


b


E


x ~O o



o



., CA 02291974 1999-12-10
' - 25 -
Example 10 Effect on mice with high blood sugar level
When streptozotocin solution (90 mg/kg) was
administered i.v. daily to mice (body weight 21-23g), the
mice became conditioned with high blood sugar, reduced
body weight and produced conditions with increasing
drinking and urine.
This'~example used mice with blood sugar level
of 180mg/dl or more. The mice (weight 21-23g) were
divided into three groups (normal mice group, mice group
with high blood sugar level and mice group with high
blood sugar level and administration of mixed extracts
(granule). They were examined for their
blood sugar levels. The results are shown in Table 10.
After the mice with high blood sugar level received
mixed extracts (granule), the high blood sugar
level became close to normal level. The mixed extracts
of the present invention (granule) can effectively
control the elevation of the blood sugar level of mice
suffering from diabetes.

. CA 02291974 1999-12-10
- 26 -



m



m



m
H .~ p b _ H
ro '-I m m N C77
, ~-1 ~ r
~ ti i~ ~ b O O
> b ~ H E ~ ~
m O U H ~ ~ O
ro ~ m tr E
o N N
0 ~ .~ +~
~ W N ~
b O
m
~ ~ ro


_ .C


m
-r1 O H y~,~ ~ O O O
rd ~ _ ~ N N
O ~ ro 3
~ m m .-t' r' o
m .~ .~;
~ ' ~ ~ +I +I +I O
ro O' ,d w ~ E ~ ~ o ~n o
O ro H E N .-
O a7 a~ C1
' ~ '~'~m H " v r1 M. h 3
+~ ~ ~ O ~ ,~ M M
3 x

m W m
~ ro O
,~



U



V7 ~ m . 01
x N U
O O O C1
Ei [Jj V



-I U O



U ~-i O


.E.~ ~ v
Q ro N N N
b E O E
~i
W C
rl ro


O E



.E.7 s


m O ~"'~ b b c~
W fx ~


. ~i ~-1O
W m
- ~: .a ~ b
o ~ v a~ c~ fl1
ro
coma


O
~ r1 ~ri+
mi ~ ~ ri ~ H
sa ~ > ~ r-I
'i~'.
>
'


3 ~ 3 ~
3


x ~


a~ ro a~ H a H a
~


E ~ ro ~ ro ro
E


O f-I O ~T O tT N


H O ~I ~ f-I~ C7
W


C7 Z C7 ~n C9 m v
O



CA 02291974 1999-12-10
_ 27 _
From the above-mentioned experiments with animals, the
following results are shown clearly.
1) The administration of 'Denhichi-Tochu-Sei" and
"Denhichi-Tochu-Sei", gallic acid-conta?nin_r~ herbal extracts
and "Licorice Root" mixed extracts show the lowering
effect of hyperlipemia in rats and also the
reduction of both serum cholesterol and lipoprotein
of low molecular weight, the slight elevation of
lipoprotein of high molecular weight and reduction
of triglycerid in the mice.
2) The administration of 'Denhichi-Tochu-Sei" results in
activation of monocyte macrophage of mice with
deteriorating immune system.
3) The long-term administration of 'Denhichi-Tochu-Sei"
is effective in enhancing the durability of mice
against fatigue.
4) The administration of "Denhichi-Tochu-Sei", gallic acid-con-
twining herbal extracts and "Licorice Root" mixed extracts are
effective in the lowering of elevated serum GTP, COT
level in rats with hepatic disease induced by D-
glucosamine.
5) The administration of "Denhichi-Tochu-Sei", gallic acid-con-
ta.in?~ herbal extracts and "Licorice Root" mixed extracts show
the inhibitory effect in IAA production of rats
induced by carbon tetrachloride, lipid-binding for
14CC1,, CO production, NADPH oxidation and oxygen
consumption.
6) The administration of 'Denhichi-Tochu-Sei" and that

CA 02291974 1999-12-10
' - 28 -
of "Denhichi-Tochu-Sei", gallic acid-containing
herbal extracts and "Licorice Root" mixed extracts
have lowered blood sugar level of rats with high
blood sugar induced by streptozotocin.
The above results show the preventive effects
of "Denhichi-Tochu-Sei" for hyperlipemia and the
significant reduction of blood sugar level by "Denhichi-
Tochu-Sei" and also "Denhichi-Tochu-Sei", "Gallae
rhois" and "Licorice Root" mixed extracts, or
taking in food and feed-with them added. And the mixed
extracts play a role in improvement of hepatic function.
These results prove that this invention can effectively
improve the health of human being and animals.
Moreover, it is noteworthy that no side effects
including acute toxic side effect were not seen in any of
the animal tests.
Example 11 Treatment effect of "Denhichi-Tochu-Sei",
"Gallae rhois" and "Licorice Root" mixed extracts
for the symptoms of patients with chronic viral hepatitis
C
A HCV patient (male, aged 50) was treated for
about 3.5 months by interferons (IFN) and no improvements
in his GOP and GTP were observed. On the other hand,
after the patient was treated for ten days by the
-administration of "Denhichi-Tochu-Sei", "Gallae
rhois" and "Licorice Root" mixed extracts, the GOP
level was improved from 159 to 89 and the GTP from 204 to


-29-

48. The results are showed in Table 11.

CA 02291974 1999-12-10
- 30 -
0


N .-1



\ p~ Op


Ov ~ ~ er


N
a


0


' '



O


m ~d



~riC, ~ ~ W er O



~ E ~ r1 N N


- ~ ~ ~ Q


x



'


c, o


\


'



'


W n o


r-1 N



d' ~


\


r1 e-i



~7 tl~


C~ C1
cd \


~-I O


E m ,1 ~""i


O~



\



O~
~i


' '~'~


O


O



O .r-IC Z .-i t~


\ , N


ao 0



G



(d



~ CO N


\ ~ er tf7



r1 N N


E W


~ ~


C H
7



E


c a
n


a a



CA 02291974 1999-12-10
- 31 -
Example 12 Treatment effect of 'Denhichi-Tochu-Sei",
"Gallae rhois" and "Licorice Root" mixed extracts,
for the patients with non-viral hepatitis
After a female patient with non-viral hepatitis
was treated for ten days by the dosage of "Denhichi-
Tochu-Sei", "Gallae rhois" and "Licorice Root"
mixed extracts, the GOP level was raduced from 53 to 39
and the GTP from 58 to 35. The results are shown in
Table 12.

CA 02291974 1999-12-10
- 32 -



b



.u o n


0


~
b


a I w


O +~ O N r0


U N +


O ri C",. U +~


N C O 'C1 c0 N


.-i ri ~-1 ~ ~I ~1



C~ ,~ ~ ld ~ X W Cv tn


cv E c~ N E ~ .. c~ c~


I w


+~ O N


- N +~ O


~r-I C. U r-1


N _ O O 'L1 O


.-I Ti ri m ~1 ~I
N


'~'


ao .C ~ M ac
cd .~I iC w ~0


ov E I~ ~ E ~ v 27 In In


e~


\


ao O n


ov n ov


O



\


n N N


c~ ~o n


M



m n n


C~ ~O C~


N -


n


\


~o o n


ov ~ er



M
O


\
E



- o~ .c o~


N


\ ~O


~O n O


o~ n


N



m n o~


O w D If7


~D


\


1n OD O~


o w n n



\ n O


_ w - w e


O W -1 ri


O



\ M d~


er O tn


C~ ~-I d'


n


\


er o0 M


c w n n


N


\ n


er d' N d'


C~ Ov e-1 C~


E W



E



E


c a
n




CA 02291974 1999-12-10
' - 33 -
Example I3 Effect of 'Denhichi-Tochu-Sei", "Gallae
rhois" and "Licorice Root" mixed extracts for the
patient with hepatitis B
A patient (male, aged 70) with HBs antibody
positive was treated for ten days with the dosage of
"Denhichi-Tochu-Sei", "Gallae rhois" and "Licorice
Root" mixed extracts. At the fourth day of the dosage,
his GOP level was lowered from 28 to 23 and the GTP from
60 to 28. The results are shown in Table 13.
Table 13
99/1 99/2


The


administ-


ration of (fourth (eighth


Hospitalized


m~ed day) day)


extracts


(first day)



AST(GOT) 55-115 82 23 27


ALT(GTP) 56-123 60 28 15


?' -GTP 33-51 35 27 24


Example 14 Effect of 'Denhichi-Tochu-Sei", "Gallae
rhois" and "Licorice Root" mixed extracts for
patients with drug hepatitis
A patient (male, aged 30) with drug hepatitis
was treated for ten days with the dosage of "Denhichi-
Tochu-Sei", "Gallae rhois" and "Licorice Root"

CA 02291974 1999-12-10
_ 34 _
mixed extracts. At the fifth day after the stop of
administration, the GOP level was lowered from 43 to 32
and the GTP from 121 to 27. The results are shown in
Table 14.
Table 14
'98/11 '99/1



Beginning The fifth


The 17th


the day of ter


day of ter


Leaving administ- the stop


the stop of


hospital ration of of


administ-


mixed administ-


ration


extracts ration



AST(GOT) 66 43 32 38


ALT(G.TP) 200 121 27 88


Example 15 Effect of "Denhichi-Tochu-Sei", "Gallae
rhois" and "Licorice Root" mixed extracts for the
symptoms of patients with chronic active hepatitis C
A patient with abnormal liver function (GOT 80,
GTP 102,?'-GTP 68) received a dosage of interferons with
three times a week for two weeks. At the eleventh day of
the dosage, side effects appeared. Thereafter, instead
~of interferons, the "Denhichi-Tochu-Sei" was given in a
dose of 6g per day for six days. The side effects
disappeared and the liver function became better (GOT 60,

CA 02291974 1999-12-10
- 35 -
GTP 47,r-GTP 17 ). However, the amount of viruses still
remained unchanged. After leaving the hospital, the
patient received interferons three times a week and about
two weeks later, "Denhichi-Tochu-Sei", "Gallae
rhois" and "Licorice Root" mixed extracts in a dose
of 3 g per day for a week. The amount of viruses was
reduced to 0.5 Meq/ml or less. The liver function
returned to the normal condition (GOT 31, GPT8,T-GTP
14). The normal liver function (GOT 25, GTP 9,?'-GTP 12)
was obtained after two months of the administration of
"Denhichi-Tochu-Sei", "Gallae rhois" and "Licorice
Root" mixed extracts. The results are showed in Table
15.
Table 15
8/19 9/11


7/29 8/18 8/25 9/18 11/24


*1 *2


AST(GOT) 80 50 60 31 25


ALT(GTP) 102 58 47 8 9


?'-GTP 68 83 17 14 12


*1 The-administration of interferons and "Denhichi-Tochu-
Sei" were started
*2 "Denhichi-Tochu-Sei", "Gallae rhois" and
'Licorice Root" mixed extracts
As explained above, "Denhichi-Tochu-Sei" and "Denhichi-
Tochu-Sei", gallic acid-containing herbal extracts and "Licorice

CA 02291974 1999-12-10
- - 36 -
Root' mixed extracts of this invention can be used as
food supplement (feed supplement) for healthcare. The
compositions of this invention are proved to be effective
in protection against hypertension and liver disorders.
They also proved to be useful for healthcare and
improvement of nutritional state and immune system.
Especially, in.case of the mixed preparation of gallic acid-
containing herbal extracts and "Licorice Root" with "Denhichi-
Tochu-Sei", both components can be easily absorbed into the
body of human being and~animals. In addition, the rapid
effects with the above-mentioned preparation are
observed.

Representative Drawing

Sorry, the representative drawing for patent document number 2291974 was not found.

Administrative Status

For a clearer understanding of the status of the application/patent presented on this page, the site Disclaimer , as well as the definitions for Patent , Administrative Status , Maintenance Fee  and Payment History  should be consulted.

Administrative Status

Title Date
Forecasted Issue Date 2003-09-30
(22) Filed 1999-12-10
Examination Requested 1999-12-10
(41) Open to Public Inspection 2001-02-23
(45) Issued 2003-09-30
Expired 2019-12-10

Abandonment History

There is no abandonment history.

Payment History

Fee Type Anniversary Year Due Date Amount Paid Paid Date
Request for Examination $200.00 1999-12-10
Registration of a document - section 124 $100.00 1999-12-10
Application Fee $150.00 1999-12-10
Maintenance Fee - Application - New Act 2 2001-12-10 $50.00 2001-11-05
Maintenance Fee - Application - New Act 3 2002-12-10 $50.00 2002-10-09
Final Fee $150.00 2003-07-08
Maintenance Fee - Patent - New Act 4 2003-12-10 $50.00 2003-10-09
Maintenance Fee - Patent - New Act 5 2004-12-10 $100.00 2004-09-15
Maintenance Fee - Patent - New Act 6 2005-12-12 $100.00 2005-09-20
Maintenance Fee - Patent - New Act 7 2006-12-11 $100.00 2006-09-28
Maintenance Fee - Patent - New Act 8 2007-12-10 $100.00 2007-09-24
Maintenance Fee - Patent - New Act 9 2008-12-10 $100.00 2008-09-16
Maintenance Fee - Patent - New Act 10 2009-12-10 $125.00 2009-11-23
Maintenance Fee - Patent - New Act 11 2010-12-10 $125.00 2010-10-20
Maintenance Fee - Patent - New Act 12 2011-12-12 $125.00 2011-10-05
Maintenance Fee - Patent - New Act 13 2012-12-10 $125.00 2012-10-03
Maintenance Fee - Patent - New Act 14 2013-12-10 $125.00 2013-10-04
Maintenance Fee - Patent - New Act 15 2014-12-10 $225.00 2014-10-09
Maintenance Fee - Patent - New Act 16 2015-12-10 $225.00 2015-11-03
Maintenance Fee - Patent - New Act 17 2016-12-12 $225.00 2016-11-21
Maintenance Fee - Patent - New Act 18 2017-12-11 $225.00 2017-11-08
Maintenance Fee - Patent - New Act 19 2018-12-10 $225.00 2018-10-02
Owners on Record

Note: Records showing the ownership history in alphabetical order.

Current Owners on Record
SHA, SHINHAN
Past Owners on Record
SHA, SHINHAN
YANAIHARA, NOBORU
Past Owners that do not appear in the "Owners on Record" listing will appear in other documentation within the application.
Documents

To view selected files, please enter reCAPTCHA code :



To view images, click a link in the Document Description column. To download the documents, select one or more checkboxes in the first column and then click the "Download Selected in PDF format (Zip Archive)" or the "Download Selected as Single PDF" button.

List of published and non-published patent-specific documents on the CPD .

If you have any difficulty accessing content, you can call the Client Service Centre at 1-866-997-1936 or send them an e-mail at CIPO Client Service Centre.


Document
Description 
Date
(yyyy-mm-dd) 
Number of pages   Size of Image (KB) 
Cover Page 2001-02-16 1 25
Abstract 2002-11-22 1 15
Description 2002-11-22 36 956
Claims 2002-11-22 5 177
Claims 2002-12-04 5 175
Cover Page 2003-08-27 1 28
Claims 2002-05-10 3 75
Abstract 1999-12-10 1 14
Description 1999-12-10 36 919
Claims 1999-12-10 2 69
Correspondence 2000-01-13 1 2
Assignment 1999-12-10 3 115
Assignment 2000-02-03 2 52
Prosecution-Amendment 2002-05-10 2 34
Prosecution-Amendment 2002-07-22 2 32
Prosecution-Amendment 2002-11-22 19 660
Prosecution-Amendment 2002-12-04 3 89
Correspondence 2003-07-08 1 34
Fees 2003-10-09 1 31
Fees 2002-10-09 1 36
Fees 2004-09-15 1 31
Fees 2001-11-05 1 29
Fees 2005-09-20 1 33
Fees 2006-09-28 1 28
Fees 2007-09-24 1 40
Fees 2007-09-24 2 57
Fees 2008-09-16 1 37
Fees 2009-11-23 1 35
Fees 2010-10-20 1 36